Stereotaxic System STS100
An easy to use and versatile stereotaxic system for surgery on mice.
The highly advanced stereotaxic system STS100 has been developed in close collaboration of scientists and lab technicians of the Center for Neurogenomics and Cognitive Research (www.cncr.nl) with staff of the fine mechanics and electronics departments of the VU University Amsterdam. The STS100 (Fig. 1) is adjustable for both right- and left-handed users. It has two arms with a drill and a pin/electrode holder that can be rotated and locked for stabile drilling and other surgical procedures.
- STS100 includes the parts needed for all typical experiments
- Ultimate stability and adjustability
- User friendliness for very fast and ultimately precise surgical procedures such as chronic cannula implantations (see References below)
- Ultimate flexibility for adjusting the system to different needs. Such as viral vector injections or in vivo electrophysiology. A variety of standard components can be added to the two arms on the same platform to meet all (advanced) experimental needs.
- The STS100 includes a heating system to maintain body temperature of anesthetized mice during the surgical procedure
- The STS100 includes a fixed drill and can be used for surgery instantly upon delivery.
Standard system components
- Stable aluminum base plate with four feet
- Two rotating arms:
Arm 1 with drill with fine and course manual Z-axis drive (dynamic range 7m)
Arm 2 with cannula fixation pin or electrode holder
- Animal platform with horizontal X- and Y-axis adjustment
- X-Y indicators with 1 µm resolution that are resettable (e.g., after drill alignment to Bregma)
- Heating block for body temperature control. Low noise temperature controller with an accuracy of +/- 0.2 0C, a resolution of 0.1°C and a maximum of 40°C. No rectal probe is required.
- Nose adaptor for stabile head fixation for use with gas anesthesia or to inject anesthesia
- Electrode holder for glass micro-electrodes (range: 0.5-2 mm diameter)
- Anesthesia gas evacuator sink box
Optional accesories for Stereotaxic System STS100:
- Optional add-on components (eg. ear bars, holder for stereo-microscope or cannula holder) can be manufactured on demand and in feedback with users.
Recommended additional equipment and its purpose (not provided)
- Cold light: proper illumination of surgical area
- Stereo-microscope: optimal coordinate positioning (e.g., Bregma), viral injection using glass micro-electrodes
- Electrically controlled micro-manipulator: for cannula insertion into defined depth
- Gas anesthesia system for optimal inhalation anesthesia (e.g. using an isoflurane vaporizer)
- Micro-infusion pump: delivery of drugs/viral solutions of defined volume and injection rate
- Additional information on components and standard operating procedures required and/or recommendable for stereotaxic surgery is available from Oliver Stiedl to customers upon request.
Stereotaxic System STS100 including standard system components (excl. VAT and delivery costs) € 19.995,-
Ir. Joost C. Rosier, Head of Fine Mechanics Instrumentation
Tel. 0031 (0) 20 598 87439
The STS100 was fundamental for experiments in mice described in these publications:
- Baarendse PJJ, van Grootheest G, Jansen RF, Pieneman AW, Ögren SO, Verhage M, Stiedl O (2008) Differential involvement of the dorsal hippocampus in passive avoidance in C57BL/6J and DBA/2J mice. Hippocampus 18, 11-19.
- Eriksson TM, Holst S, Stan TL, Hager T, Sjögren B, Ögren SO, Svenningsson P, Stiedl O (2012) 5-HT1A and 5-HT7 receptors crosstalk in the regulation of emotional learning: implications for the mechanisms of selective serotonin reuptake inhibitors. Neuropharmacology 63, 1150-1160.
- Misane I, Kruis A, Pieneman AW, Ögren SO, Stiedl O (2013) GABA. A receptor activation in the CA1 area of the dorsal hippocampus impairs conditioned contextual fear in C57BL/6J mice. Behavioural Brain Research 238, 160-169.
- Rao-Ruiz P, Rotaru DC, van der Loo R, Mansvelder H, Stiedl O, Smit AB, Spijker S (2011) Dorsohippocampal biphasic expression of GluA2-containing AMPARs is critical for reconsolidation-update of emotional memories. Nature Neuroscience 14, 1302-1308.
- Van den Oever MC, Rotaru DC, Heinsbroek JA, Yvonne Gouwenberg Y, Deisseroth K, Stuber GD, Mansvelder HD, Smit AB (2013) Ventromedial prefrontal cortex pyramidal cells have a temporal dynamic role in recall and extinction of cocaine-associated memory. Journal of Neuroscience 33, 18225-18233.